Quiznetik
Biotechnology | Set 1
1. Restriction enzymes were discovered by
A. Smith and Nathans
B. Alexander Fleming
C. Berg
D. None
Correct : A. Smith and Nathans
2. Bacteria protect themselves from viruses by fragmenting viral DNA with
A. Ligase
B. Endonuclease
C. Exonuclease
D. Gyrase
Correct : B. Endonuclease
3. Klenow fragment is derived from
A. DNA Ligase
B. DNA Pol-I
C. DNA Pol-II
D. Reverse Transcriptase
Correct : B. DNA Pol-I
4. Southern blotting is
A. Attachment of probes to DNA fragments
B. Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
C. Comparison of DNA fragments to two sources
D. Transfer of DNA fragments to electrophoretic gel from cellulose membrane
Correct : B. Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
5. ELISA is
A. Using radiolabelled second antibody
B. Usage of RBCs
C. Using complement-mediated cell lysis
D. Addition of substrate that is converted into a coloured end product
Correct : D. Addition of substrate that is converted into a coloured end product
6. The Golden Rice variety is rich in
A. Vitamin C
B. Β-carotene and ferritin
C. Biotin
D. Lysine
Correct : B. Β-carotene and ferritin
7. The DNA fragments have sticky ends due to
A. Endonuclease
B. Unpaired bases
C. Calcium ions
D. Free methylation
Correct : B. Unpaired bases
8. Plasmids are used as cloning vectors for which of the following reasons?
A. Can be multiplied in culture
B. Self-replication in bacterial cells
C. Can be multiplied in laboratories with the help of enzymes
D. Replicate freely outside bacterial cells
Correct : B. Self-replication in bacterial cells
9. The human genome project was launched in the year
A. 1980
B. 1973
C. 1990
D. 1989
Correct : C. 1990
10. The vaccines prepared through recombinant DNA technology are
A. Third generation vaccines
B. First-generation vaccines
C. Second-generation vaccines
D. None
Correct : A. Third generation vaccines
11. Which is a genetically modified crop?
A. Bt-cotton
B. Bt-brinjal
C. Golden rice
D. All
Correct : D. All
12. PCR technique was invented by
A. Karry Mullis
B. Boyer
C. Sanger
D. Cohn
Correct : A. Karry Mullis
13. The first transgenic plant to be produced is
A. Brinjal
B. Tobacco
C. Rice
D. Cotton
Correct : B. Tobacco
14. RNA interference helps in
A. Cell proliferation
B. Micropropagation
C. Cell defence
D. Cell differentiation
Correct : C. Cell defence
15. Which of the following is the quality of improved transgenic basmati rice?
A. Gives high yield but no characteristic aroma
B. Gives high yield and is rich in vitamin A
C. Does not require chemical fertilizers and growth hormones
D. Resistant to insects and diseases
Correct : B. Gives high yield and is rich in vitamin A
16. The first clinical application of gene therapy over a 4 year old girl was for
A. Adenosine deaminase deficiency
B. Adenosine deficiency
C. Growth deficiency
D. Adenine deficiency
Correct : A. Adenosine deaminase deficiency
17. Excision and insertion of a gene is called
A. Biotechnology
B. Genetic engineering
C. Cytogenetics
D. Gene therapy
Correct : B. Genetic engineering
18. The expression of a transgene in the target tissue is identified by a
A. Transgene
B. Promoter
C. Enhancer
D. Reporter
Correct : D. Reporter
19. ———– is used as a vector for cloning into higher organisms
A. Retrovirus
B. Baculovirus
C. Salmonella typhimurium
D. Rhizopus nigricans
Correct : A. Retrovirus
20. Which bacterium is used in the production of insulin by genetic engineering?
A. Saccharomyces
B. Rhizobium
C. Escherichia
D. Mycobacterium
Correct : C. Escherichia
21. The Taq polymerase enzyme is obtained from
A. Thermus aquaticus
B. Thiobacillus ferrooxidans
C. Bacillus subtilis
D. Pseudomonas subtilis
Correct : A. Thermus aquaticus
22. Which of the following is an endonuclease?
A. DNase I
B. Hind II
C. Protease
D. RNase restriction
Correct : B. Hind II
23. Which of the following restriction enzymes produce blunt ends?
A. Hind III
B. ho
C. Eco RV
D. Sal I
Correct : C. Eco RV
24. Which of the following is not a component of downstream processing?
A. Expression
B. Preservation
C. Purification
D. Separation
Correct : A. Expression
25. A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
A. Taq polymerase
B. Polymerase III
C. Ligase
D. Eco RI
Correct : C. Ligase
26. DNA fragments separated on an agarose gel can be visualized after staining with ____
A. ethidium bromide
B. bromophenol blue
C. acetocarmine
D. aniline blue
Correct : A. ethidium bromide
27. A gene whose expression helps to identify transformed cells is known as ____
A. Plasmid
B. Selectable marker
C. Structural gene
D. vector
Correct : B. Selectable marker
28. A single strand of nucleic acid tagged with a radioactive molecule is called
A. Plasmid
B. Probe
C. selectable marker
D. Vector
Correct : B. Probe
29. There is a restriction endonuclease called Eco RI. What does ‘co’ part in it stand for?
A. Coli
B. Colon
C. Cofactor
D. None of the above
Correct : A. Coli
30. Agarose extracted from sea weeds finds use in ____
A. Spectrophotometry
B. Gel electrophoresis
C. PCR
D. tissue culture
Correct : B. Gel electrophoresis
31. Animal biotechnology involves
A. production of valuable products in animals using rDNA technology
B. rapid multiplication of animals of desired genotypes
C. alteration of genes to make it more desirable
D. all of these
Correct : D. all of these
32. Animal cell cultures are used widely for the production of
A. insulin
B. somatostatin
C. mabs
D. thyroxine
Correct : C. mabs
33. The first vaccine developed from animal cell culture was
A. Hepatitis B vaccine
B. Influenza vaccine
C. Small pox vaccine
D. Polio vaccine
Correct : D. Polio vaccine
34. Which of the following are commonly produced in animal cell cultures
A. Interferon
B. mab
C. vaccines
D. all of these
Correct : D. all of these
35. The cell line used for the production of polio vaccine was
A. Primate kidney cell line
B. CHO cell line
C. Dog kidney cell line
D. mouse fibroblast cell line
Correct : A. Primate kidney cell line
36. Recombinant proteins are
A. proteins synthesized in animals
B. proteins synthesized by transgene in host cell by rDNA technology
C. proteins synthesised in cells that are produced by protoplast fusion
D. proteins synthesized in mutated cell lines
Correct : B. proteins synthesized by transgene in host cell by rDNA technology
37. Interferons are
A. anti bacterial proteins
B. anti-viral proteins
C. bacteriostatic proteins
D. all of these
Correct : B. anti-viral proteins
38. The virus commonly used to infect cell cultures for the production of interferon is
A. Corona virus
B. Sendai virus
C. Polio virus
D. Small pox virus
Correct : B. Sendai virus
39. Hybrid antibodies are
A. antibodies produced in cell cultures
B. antibodies designed using rDNA technology produced in cell cultures
C. antibodies produced in invivo
D. both a and b
Correct : B. antibodies designed using rDNA technology produced in cell cultures
40. The technique used in animal biotechnology for the rapid multiplication and production of animals with a desirable genotype is
A. protoplast fusion and embryo transfer
B. hybrid selection and embryo transfer
C. in vitro fertilization and embryo transfer
D. all of these
Correct : C. in vitro fertilization and embryo transfer
41. The production of complete animals from somatic cells of an animal is called
A. gene cloning
B. animal cloning
C. cell cloning
D. all of these
Correct : B. animal cloning
42. The first successfully cloned animal was
A. monkey
B. gibbon
C. sheep
D. rabbit
Correct : C. sheep
43. In humans, the babies produced by in vitro fertilization and embryo transfer was popularly called as
A. invitro babies
B. test tube babies
C. invitro-invivo babies
D. all of these
Correct : B. test tube babies