Quiznetik

Biotechnology | Set 1

1. Restriction enzymes were discovered by

Correct : A. Smith and Nathans

2. Bacteria protect themselves from viruses by fragmenting viral DNA with

Correct : B. Endonuclease

3. Klenow fragment is derived from

Correct : B. DNA Pol-I

4. Southern blotting is

Correct : B. Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet

5. ELISA is

Correct : D. Addition of substrate that is converted into a coloured end product

6. The Golden Rice variety is rich in

Correct : B. Β-carotene and ferritin

7. The DNA fragments have sticky ends due to

Correct : B. Unpaired bases

8. Plasmids are used as cloning vectors for which of the following reasons?

Correct : B. Self-replication in bacterial cells

9. The human genome project was launched in the year

Correct : C. 1990

10. The vaccines prepared through recombinant DNA technology are

Correct : A. Third generation vaccines

11. Which is a genetically modified crop?

Correct : D. All

12. PCR technique was invented by

Correct : A. Karry Mullis

13. The first transgenic plant to be produced is

Correct : B. Tobacco

14. RNA interference helps in

Correct : C. Cell defence

15. Which of the following is the quality of improved transgenic basmati rice?

Correct : B. Gives high yield and is rich in vitamin A

16. The first clinical application of gene therapy over a 4 year old girl was for

Correct : A. Adenosine deaminase deficiency

17. Excision and insertion of a gene is called

Correct : B. Genetic engineering

18. The expression of a transgene in the target tissue is identified by a

Correct : D. Reporter

19. ———– is used as a vector for cloning into higher organisms

Correct : A. Retrovirus

20. Which bacterium is used in the production of insulin by genetic engineering?

Correct : C. Escherichia

21. The Taq polymerase enzyme is obtained from

Correct : A. Thermus aquaticus

22. Which of the following is an endonuclease?

Correct : B. Hind II

23. Which of the following restriction enzymes produce blunt ends?

Correct : C. Eco RV

24. Which of the following is not a component of downstream processing?

Correct : A. Expression

25. A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

Correct : C. Ligase

26. DNA fragments separated on an agarose gel can be visualized after staining with ____

Correct : A. ethidium bromide

27. A gene whose expression helps to identify transformed cells is known as ____

Correct : B. Selectable marker

28. A single strand of nucleic acid tagged with a radioactive molecule is called

Correct : B. Probe

29. There is a restriction endonuclease called Eco RI. What does ‘co’ part in it stand for?

Correct : A. Coli

30. Agarose extracted from sea weeds finds use in ____

Correct : B. Gel electrophoresis

31. Animal biotechnology involves

Correct : D. all of these

32. Animal cell cultures are used widely for the production of

Correct : C. mabs

33. The first vaccine developed from animal cell culture was

Correct : D. Polio vaccine

34. Which of the following are commonly produced in animal cell cultures

Correct : D. all of these

35. The cell line used for the production of polio vaccine was

Correct : A. Primate kidney cell line

36. Recombinant proteins are

Correct : B. proteins synthesized by transgene in host cell by rDNA technology

37. Interferons are

Correct : B. anti-viral proteins

38. The virus commonly used to infect cell cultures for the production of interferon is

Correct : B. Sendai virus

39. Hybrid antibodies are

Correct : B. antibodies designed using rDNA technology produced in cell cultures

40. The technique used in animal biotechnology for the rapid multiplication and production of animals with a desirable genotype is

Correct : C. in vitro fertilization and embryo transfer

41. The production of complete animals from somatic cells of an animal is called

Correct : B. animal cloning

42. The first successfully cloned animal was

Correct : C. sheep

43. In humans, the babies produced by in vitro fertilization and embryo transfer was popularly called as

Correct : B. test tube babies